Nonstructural protein of infectious bursal disease virus inhibits apoptosis at the early stage of virus infection.

Early Stage of Virus Infection Disease Virus Inhibits Apoptosis at the Nonstructural Protein of Infectious Bursal

An experimental study on early pathogenesis of a very mvirulent isolate of infectious bursal disease virus, employing immunohistochemistry

In this study, immunohistochemistry was used to clarify the early stages of viral kinetics and cycliccourse of IBDV, IR499, which has been described earlier as a very virulent strain (vvIBDV). Fifteen, 4-week-old SPF chickens were inoculated with 10³ EID50 of vvIBDV, IR499, via oculo/nasal route. Five birdsserved as controls, and inoculated with phosphate buffered saline (PBS). The birds were t...

Cloning and Expression of VP2 Gene of Infectious Bursal Disease Virus in Eukaryotic Cells

Infectious bursal disease (IBD) is an economically important viral disease of chickens with worldwide distribution which suppresses the immune system of young chickens. VP2 is the major host-protective protein of infectious bursal disease virus (IBDV). The encoding region of VP2 protein was PCR amplified from a plasmid containing a cDNA fragment of large genomic segment of IBDV, strain D78. Thi...

Crude extract of bacterially expressed, infectious bursal disease virus VPX/VP2 proteins induces protective immune response to the virus in chickens

Infectious bursal disease virus (IBDV) infects young chickens and causes serious lose to the poultry industry, worldwide. Previous attempts using purified bacterially expressed IBDV VP2 failed to elicit a protective immune response to the virus. This study was designed to investigate if the initial expressed protein contained neutralizing epitopes but became nonfunctional during purification st...

Expression of the VP2 gene of classical D78 infectious bursal disease virus in the methylotrophic yeast Pichia pastoris as a secretory protein

Infectious bursal disease virus (IBDV) is the causative agent of Gumboro disease, an infectious disease of global economic importance in poultry. The expression of heterologous proteins in P.pastoris is fast, simple and inexpensive. In this study, VP2 encoding gene of classical D78 IBDV was amplified using reverse transcription (RT) polymerase chain reaction (PCR) and cloned into pPICZαA vector...